An article in Bioresource Technology [Preliminary Tests on Nisin and Pediocin Production Using Waste Protein Sources: Factorial

An article in Bioresource Technology [€œPreliminary Tests on Nisin and Pediocin Production Using Waste Protein Sources: Factorial and Kinetic Studies€ (2006, Vol. 97(4))] described an experiment in which pediocin was produced from waste protein. Nisin and pediocin are bacteriocins (compounds produced by bacteria that inhibit related strains) used for food preservation. Three levels of protein (g/L) from trout viscera extracts were compared.

(a) Construct box plots of the data. What visual impression do you have from these plots?

(b) Does the level of protein have an effect on mean pediocin production? Use α = 0.05.

(c) Would you draw a different conclusion if α = 0.01 had been used?

(d) Plot the residuals from the experiment and comment. In particular, comment on the normality of the residuals.

(e) Find a 95% confidence interval on mean pediocin production when the level of protein is 2.50 g/L.

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Pediocin production (ratio to baseline) Protein - (g/L) 1.67 2.90 2 3 4 5 6 1 8 9 3.42 3.18 3.25 10 2.50 2.64 4.52 2.95 4.35 4.65 4.54 4.29 4.42 4.47 3.98 3.33 3.77 4.5 4.62 4.87