The enzyme lactate dehydrogenase catalyzes the reaction NADH absorbs light at 340 nm in the near-ultraviolet region

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The enzyme lactate dehydrogenase catalyzes the reactionPyruvate + NADH+H*lactate + NAD+

NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic spectrum, but NAD+ does not. Suggest an experimental method for following the rate of this reaction, assuming that you have available a spectrophotometer capable of measuring light at this wavelength.

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Biochemistry

ISBN: 9781305961135

9th Edition

Authors: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

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