Question: Repeat the above problem, except this time solve by the differential method. Data from above problem At room temperature sucrose is hydrolyzed by the catalytic

Repeat the above problem, except this time solve by the differential method.

Data from above problem

At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows:sucrose sucrase products

Starting with a sucrose concentration CA0 = 1.0 millimol/liter and an enzyme concentration CEO = 0.01 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements):

CA, millimol/liter t, hr 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 1 2 3 4 5 6 7 8 9 10 11

Determine whether these data can be reasonably fitted by a kinetic equation of the Michaelis-Menten type, or

-TA = K3CA CEO CA + CM where CM Michaelis constant =

If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.

sucrose sucrase products

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