The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:

(a) Construct a spreadsheet, and use it to draw a calibration curve for NADH.
(b) Find the least-squares slope and intercept for the plot in (a).
(c) Calculate the standard deviation of the slope and the standard deviation about regression for the curve.
(d) An unknown exhibits a relative fluorescence intensity of 11.34. Use the spreadsheet to calculate the concentration of NADH.
(e) Calculate the relative standard deviation for the result in part (d).
(f) Calculate the relative standard deviation for the result in part (d) if the reading of 12.16 was the mean of three measurements.

Transcribed Image Text:

Concn NADH, Relative umol/L Intensity 0.100 2.24 0.200 4.52 0.300 6.63 0.400 9.01 0.500 10.94 0.600 13.71 15.49 0.700 0.800 17.91