Will the bright pixel tracing function only work in darkness? Make your own movie with someone writing

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Will the bright pixel tracing function only work in darkness? Make your own movie with someone writing with a glow stick or flashlight, in normal brightness. Does the function still work? Do you need to use a different threshold value? Do you have to try a different approach to identify “bright” pixels (e.g., perhaps by comparing luminance a particular pixel to the pixels around it)?

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