Question: Preparing plasmid (double-stranded, circular) DNA for sequencing involves annealing a complementary, short, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is

Preparing plasmid (double-stranded, circular) DNA for sequencing involves annealing a complementary, short, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90^oC and then slowly bringing the temperature down to 25^oC. Why does this protocol work?

Step by Step Solution

★★★★★

3.39 Rating (158 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

At 90C the doublestranded DNA template will denature and the strands will separate As the tempera... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (2 attachments)

1624_606b0df17cd92_649082.pdf

180 KBs PDF File

1624_606b0df17cd92_649082.docx

120 KBs Word File

Students Have Also Explored These Related Molecular Cell Biology Questions!

Ten micrograms of a decanucleotide-pair HpaI restriction fragment were isolated from the double-stranded DNA chromosome of a small virus. Octanucleotide poly(A) tails were then added to the 3²...

As discussed in Chapter 17, some viruses contain RNA as their genetic material. Certain RNA viruses can exist as a provirus in which the viral genetic material has been inserted into the chromosomal...

Te gel electrophoresis pattern in Figure 4.23 was determined by soaking the gel in a solution of ethidium bromide (EtBr). This is a uorescent molecule with a planar structure: Te at molecule...

A bacterial genome ( double - stranded circular DNA ) contains 7 % thymine. What percent cytosine does it contain? Express your answer as a percent, but leave the sign off ( 2 5 for 2 5 % , and not 0...

Bacterial chromosomes are composed of double - stranded circular DNA. True False

Question: Genomic sequence of gene of interest:...

please answer questions 2,4,7 and 8 ii) The percentage of agarose used to separate plasmid is determined by iii) How many grams of agarose do you need to make 50mL of a 1% agarose solution? 0.3g iv)...

Question 4 2 1 pts What is the structure of plasmid DNA? Single - stranded circular Double - stranded circular Single - stranded linear Double - stranded linear

What is the structure of plasmid DNA? Single - stranded circular Double - stranded circular Single - stranded linear Double - stranded linear

i) What does agarose do in the agarose gel electrophoresis? ii) The percentage of agarose used to separate plasmid is determined by iii) How many grams of agarose do you need to make 50mL of a 1%...

Consider an experiment with three groups, with seven values in each. a. How many degrees of freedom are there in determining the among-group variation? b. How many degrees of freedom are there in...

The region bounded by y = coshx, y = 0, x = 0 and x = 1 is revolved about the x-axis. Find the volume of the resulting solid. cosh2 x = (1 + cosh2x)/2

show the formula to calculate the least square fit. MATLAB

134. In Problem 132, what uniform annual investment is required to achieve the same account balance?

Now that you know the dimensions of the mural and the magnification that accommodates the largest trees, what size would a 1-foot-long reptile be in the mural?

Artists often use insets (small internal drawings) to change the magnification of a portion of a larger drawing. If you wish to show this reptile and its 1-inch-long egg, what magnification might you...

Next examine the following figure. How does the density of human cases compare with the distribution of the H5N1 flu in poultry? In wild birds? LNITID KNDOM OF GEAT IAN AND MO ELAN DEM Jowib UAN...

YTM= 8.57% RISK FREE RATE = 1.3% BETA = 3.13 Ch 14: Using the Risk Free rate from above, and assuming a 4 percent market risk premium a) What is the cost of equity for your selected company using...

Suppose we have the following returns for large-company stocks and Treasury bills over a six year period: Year Large Company US Treasury Bill 1 3.96 4.50 2 14.12 4.88 3 19.01 3.80 4 14.67 6.96 5...

The Stata data file (elemapi.dta) contains data on 21 school-related variables in 400 U.S. schools. Please refer to the text file (elemapi description.txt) for a description of these variables. (a)...