Ten micrograms of a decanucleotide-pair HpaI restriction fragment were isolated from the double-stranded DNA chromosome of a
Question:
5€²-X X X X X X X X X X-3€²
3€²-X€² X€² X€² X€² X€² X€² X€² X€² X€² X€²-5€²
†“ terminal transferase, dATP
5€²-X X X X X X X X X X A A A A A A A A-3€²
3€²-A A A A A A A A X€² X€² X€² X€² X€² X€² X€² X€² X€² X€²-5€²
where X and X€² can be any of the four standard nucleotides, but X€² is always complementary to X.
The two complementary strands (€œWatson€ strand and €œCrick€ strand) were then separated and sequenced by the 2€²,3€²-dideoxyribonucleoside triphosphate chain-termination method. The reactions were primed using a synthetic poly(T) octamer; that is, Watson strand
3€²-A A A A A A A A X€² X€² X€² X€² X€² X€² X€² X€² X€² X€²-5€²
5€²-T T T T T T T T-OH
Crick strand
5€²-X X X X X X X X X X A A A A A A A A-3€²
HO-T T T T T T T T-5€²
Two DNA sequencing reactions were carried out.
Reaction 1 contained the Watson strand template/primer shown above; reaction 2 contained the Crick strand template/primer. Both sequencing reactions contained DNA polymerase and all other substrates and components required for DNA synthesis in vitro plus the standard four 2€²,3€²-dideoxyribonucleoside triphosphate chain-terminators€”ddGTP, ddCTP, ddATP, and ddTTP€”each labeled with a different fluorescent dye. The dyes fluoresce at different wavelengths, which are recorded by a photocell as the products of the reactions are separated by capillary gel electrophoresis. In the standard sequencing reaction, the chains terminating with ddG fluoresce dark blue (peaks appear black in the computer printouts), those terminating with ddC fluoresce light blue, those terminating with ddA fluoresce green, and those terminating with ddT fluoresce red. The computer printout for sequencing reaction 1, which contained the Watson strand as template, is shown as follows.
Draw the predicted computer printout for reaction 2, which contained the Crick strand as template, in the following box. Remember that all DNA synthesis occurs in the 5€² †’ 3€² direction and that the sequence of the nascent strand reads 5€² to 3€² from left to right in the printout.
Fantastic news! We've Found the answer you've been seeking!
Step by Step Answer:
Nuc...View the full answer
Answered By
Shameen Tahir
The following are details of my Areas of Effectiveness. The following are details of my Areas of Effectiveness English Language Proficiency, Organization Behavior , consumer Behavior and Marketing, Communication, Applied Statistics, Research Methods , Cognitive & Affective Processes, Cognitive & Affective Processes, Data Analysis in Research, Human Resources Management ,Research Project,
Social Psychology, Personality Psychology, Introduction to Applied Areas of Psychology,
Behavioral Neurosdence , Historical and Contemporary Issues in Psychology, Measurement in Psychology, experimental Psychology,
Business Ethics Business Ethics An introduction to business studies Organization & Management Legal Environment of Business Information Systems in Organizations Operations Management Global Business Policies Industrial Organization Business Strategy Information Management and Technology Company Structure and Organizational Management Accounting & Auditing Financial Accounting Managerial Accounting Accounting for strategy implementation Financial accounting Introduction to bookkeeping and accounting Marketing Marketing Management Professional Development Strategies Business Communications Business planning Commerce & Technology Human resource management General Management Conflict management Leadership Organizational Leadership Supply Chain Management Law Corporate Strategy Creative Writing Analytical Reading & Writing Other Expertise Risk Management Entrepreneurship Management science Organizational behavior Project management Financial Analysis, Research & Companies Valuation And any kind of Excel Queries.
16+ Reviews
34+ Question Solved
Related Book For 
Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons
Question Posted:
