A peptide P with the amino acid composition (A,E,F,G 2 ,H,K,L,M,P,R,V,Y) did not yield to MSMS sequencing,


A peptide P with the amino acid composition (A,E,F,G2,H,K,L,M,P,R,V,Y) did not yield to MS–MS sequencing, so it was hydrolyzed with trypsin to three peptides, T1, T2, and T3, which were found by MS–MS to have the following structures:TI: A-H-K T2: E-M-V T3: (L,P)-F-G-G-Y-R

(The parentheses in T3 mean that the order of L and P could not be determined.) Hydrolysis of P catalyzed by chymotrypsin yielded three peptides, C1, C2, and C3, which were sequenced by MS–MS and found to have the following structures:CI: A-H-K-L-P-F C2: G-G-Y C3: R-E-M-V

What is the amino acid sequence of peptide P?

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Organic Chemistry

ISBN: 9781936221349

6th Edition

Authors: Marc Loudon, Jim Parise

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