AQC-tryptophan is not shown in Fig. 27.5 because the indole ring does not survive the acid hydrolysis.
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AQC-tryptophan is not shown in Fig. 27.5 because the indole ring does not survive the acid hydrolysis. In what general region of the chromatogram would you expect to find AQC-Trp if it were present? Explain.
Transcribed Image Text:
0 5 10 15 retention time, minutes 20 25 30 35 20 AMQ relative fluorescence Cys Asp Ser Glu Gly NH3 His Arg Thr Pro Lys 60 Ala Tyr 80 Met volume % acetonitrile Val 8 lle Leu 100 Phe
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The indole sidechain of tryptophan is very h...View the full answer
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