DNA is capable of forming complex helical structures. An unusual triple-helix structure of poly(dA).2poly(dT) DNA was studied by P. V. Scaria and R. H. Shafer [Journal of Biological Chemistry 266 (1991): 5417] where the intercalation of ethidium bromide was studied using UV absorption and circular dichroism spectroscopy. The following representative data were obtained using the results of this study:

Using the data, determine K and N for the binding of ethidium bromide to the DNA triple-helical structure.

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CEch (HM) 10.0 14.7 0.63 1.7 5.0 0.007 0.039 0.059 0.070 0.017