The protein lysozyme unfolds at a transition temperature of 75.5 °C and the standard enthalpy of transition is 509 kJmol⁻¹. Calculate the entropy of unfolding of lysozyme at 25.0 °C, given that the difference in the constant pressure heat capacities upon unfolding is 6.28 kJK⁻¹mol⁻¹ and can be assumed to be independent of temperature. Imagine that the transition at 25.0 °C occurs in three steps: (i) heating of the folded protein from 25.0 °C to the transition temperature, (ii) unfolding at the transition temperature, and (iii) cooling of the unfolded protein to 25.0 °C. Because the entropy is a state function, the entropy change at 25.0 °C is equal to the sum of the entropy changes of the steps.