a. Use Figure 25-31 to select a tetrahydrofuran/water tetrahydrofuran/water mobile phase strength equivalent to the strength of
Question:
a. Use Figure 25-31 to select a tetrahydrofuran/water tetrahydrofuran/water mobile phase strength equivalent to the strength of 80% 80% methanol.
b. Describe how to prepare 1 liter of this tetrahydrofuran mobile phase.
c. What limitations would be imposed by the use of tetrahydrofuran?
Figure 25-31
Transcribed Image Text:
Acetonitrile/H₂O 0 10 20 0 Methanol/H₂O + 0 Tetrahydrofuran/H₂O H 20 methanol or 22 vol%4 30 40 40 + # + 1 10 20 30 50 + 60 60 70 80 90 60 80 + + Harris/Lucy, Quantitative Chemical Analysis, 10e, © 2020 W. H. Freeman and Company 80 + + 40 50 100 100 70 90 + 100 FIGURE 25-31 Nomograph showing volume percentage of solvents having the same mobile phase strength. A vertical line intersects each solvent line at the same eluent strength. For example, 30 vol% acetonitrile/70 vol% 30 vol% acetonitrile/70 val% water has about the same eluent strength as 40 vol%40 vol% 22 vol% tetrahydrofuran.
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Related Book For
Quantitative Chemical Analysis
ISBN: 9781319164300
10th Edition
Authors: Daniel C. Harris, Charles A. Lucy
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