Consider the separation of inorganic and organic anions in Figure 26-7. a. What is the probable charge
Question:
Consider the separation of inorganic and organic anions in Figure 26-7.
a. What is the probable charge (Xn-) (Xn-)of pyruvate (peak 1010), 2-2-oxovalerate (peak 1616), and maleate (peak 2828)?
b. Iodide (peak 44 44) is a -1-1 ion. Explain its strong retention.
Figure 26-7
Transcribed Image Text:
Conductivity (us) 31 3 T 50 2 3 A 6 9 10 11 15 12 17 ili 10 19 18 20 22 21 23 27 24 26 29 28 31 . (39) citrate, (40) Cro42 Cro. 30 32 (35) Mo042- 33 34 35 36 37 1 30 20 Time (min) Harris/Lucy, Quantitative Chemical Analysis, 10e, © 2020 W. H. Freeman and Company 39 38 40 41 43 44 FIGURE 26-7 Anion separation by ion chromatography with a gradient of electrolytically generated K+OH KOH and conductivity detection after suppression. Column: Thermo lonPac AS11-HC, 25×0.04 cm, 25 x 0.04 cm, 4-um4 4- particles, flow-15 µl/min flow = 15 L/min, eluent: 11 to 14 mM 14 M OH-OH from 0 to 16 min, 18 min, 1414 to 55 mM55 mM OH-OH from 1616 to 40 min, 40 min, injection 0.4 injection = 0.4 μL of 0.60.6 to 4 ppm4 ppm standards; peaks: (1) quinate, (2) (3) acetate, (4) lactate, (5) 2-2-hydroxybutyrate, (6) propionate, (7) formate, (8) butyrate, (9) 2-2- F hydroxyvalerate, (10) pyruvate, (11) isovalerate, (12) C102 CIO₂, (13) valerate, (14) Bro3_BrO (15) CH CI, (16) 2-2-oxovalerate, (17) NO2 NO₂ (18) ethylphosphonate, (19) trifluoroacetate, (20) azide, (21) Br NO (23) citramalate, (24) malate, (25) CO32- CO (30) a-ketoglutarate, a-ketoglutarate, wo²- Br (22) NO3-, (28) maleate, (29) SO42-,- fumarate, (33) oxaloacetate, (34) WO42, AsO SO² Mo0² (38) As 043- (44)1-I. 40 (26) malonate, (27) citraconitate, (31) C2042-C₂0² * (32) PO³ 1 (36) PO43- * (41) iso-citrate, (42) cis-aconitate, (43) trans-aconitate, > (37) phthalate,
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Related Book For
Quantitative Chemical Analysis
ISBN: 9781319164300
10th Edition
Authors: Daniel C. Harris, Charles A. Lucy
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