In Figure 22-14, the sucralose species with a nominal mass X=395 X=395 is [12C121H1816O835C13]-. [¹²C₁₂¹H₁₈¹⁶O₈³⁵Cl₃]-.  X+1X + 1 arises from isotopologues containing one 13C,¹³C, one 2H,²H, or one 17O.¹⁷O. The predicted intensity at X+1X+1 is 12x1.08%+18x0.012%+8x0.038%=13.5% 12 x 1.08%+18 x 0.012%4-8 x 0.038%= 13.5% of X.X. Use factors in Table 22-2 to write the analogous equation for the intensity at X+2 X + 2 arising from just [13C212C101H1816O835C13]-[¹³C₂¹²C₁₀¹H₁₈¹⁶O₈³⁵Cl₃]- and [12C121H1818O116O735C13]-.[¹²C₁₂¹H₁₈¹⁸O₁¹⁶O₇³⁵Cl₃]^-. Why is the intensity at X+2 X+2 so much greater than what you calculate?

Figure 22-14

Table 22-2

Transcribed Image Text:

397.099 4 OH OH Sucralose [M-HJ TIE C12H18Og Cl m/z 395.007 3 399.028 2 396.012 0 398.011 7 396 397 398 399 X+1 X+2 X+3 35C1₂37 Cl 35C³7C1₂ Relative abundance 100 80 60 40 20 0 394 395.100 0 395 X 35C13 OH m/z X+4 HO 400.003 0 401.002 4 400 OH X+5 401 X+6 37 Cl3 CI 402