Stern-Volmer plot. The table shows intensity and lifetime data for luminescence of the ruthenium complex in Figure
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Stern-Volmer plot. The table shows intensity and lifetime data for luminescence of the ruthenium complex in Figure 19-21 for a range of oxygen concentrations.
a. Why does the luminescence intensity decrease as the oxygen concentration increases?
b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear?
c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?
Figure 19-21
Transcribed Image Text:
Concentration oxygen (UM)(M) 00 2222 4444 7272 132132 174174 267267 Fluorescence intensity 590590 574574 557557 552552 518518 503503 478478 Fluorescence lifetime (us) (μs) 1.0081.008 0.9780.978 0.9640.964 0.9500.950 0.9160.916 0.8930.893 0.8360.836
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Related Book For
Quantitative Chemical Analysis
ISBN: 9781319164300
10th Edition
Authors: Daniel C. Harris, Charles A. Lucy
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