Question: A single-stranded template is properly base paired with a short primer as shown below. Assume that there is a 3'0H end in the correct place.

A single-stranded template is properly base paired with a short primer as shown below. Assume that there is a 3'0H end in the correct place. If DNA polymerase and dNTPs are added, how long will the top strand be after the enzyme has synthesized as much as it is able to? (Hint: Pay attention to the polarity of the template strand.) TTTTT GTTCT ACCGTCCAAGACCTTCAGTA | | . . . . . . .

  • 5 bases (no synthesis occurs)
  • 9 bases
  • 14 bases
  • 11 bases
  • 6 bases

TTTTT GTTCT ACCGTCCAAGACCTTCAGTA | | . . . . . . . . . . . . . . . . . 5' 3'

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