From the table below, identify what would the first protein to be eluted from each of the following methods given the conditions indicated. Size exclusion column (proteins larger than 80 kDa excluded)

Cation Exchange column (pH 7) ??????..D Anion Excahnge column (pH 5) ??????..Choose An anion exchange column (pH 6) that also separated based on molecular weight (proteins 60 kDa or greater excluded) ??????..Choose A cation exchange column (pH 6) that also separated based on molecular weight (proteins 100 kDa or greater excluded) ??????..Choose A Mg2+ affinity binding column ??????..Choose

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Protein pl A 4.5 B 6.7 C 10 D 8.5 MW (kDa) 26 58 100 26 Ligand Mg 2+ Mg2+ Mg Protein pl A 4.5 B 6.7 C 10 D 8.5 MW (kDa) 26 58 100 26 Ligand Mg 2+ Mg2+ Mg

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1 In size exclusion chromatography larger molecules are eluted first Sin... View the full answer