Some clones from cDNA libraries can have defects because of the way a cDNA library is constructed. For each of the dilemmas described in A?D, indicate which of the outcomes listed in 1?4 you might encounter. Outcomes may be used more than once.

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Dilemma A. The mRNA corresponding to the clone you are looking for was degraded at its 5' end by a nuclease. B. The mRNA corresponding to the clone you are looking for was degraded at its 3' end by a nuclease. C. The 5' end of the reverse transcriptase product of the gene you are trying to clone hybridizes to sequences in the middle of the gene. D. The gene you are trying to clone has a long stretch of A's in the middle of the coding sequence. Outcomes 1. The 5' part of the gene will be 5º missing. 2. The 3' part of the gene will be missing. An internal fragment of the gene will be missing. 4. The gene will be missing from the library. Dilemma A. The mRNA corresponding to the clone you are looking for was degraded at its 5' end by a nuclease. B. The mRNA corresponding to the clone you are looking for was degraded at its 3' end by a nuclease. C. The 5' end of the reverse transcriptase product of the gene you are trying to clone hybridizes to sequences in the middle of the gene. D. The gene you are trying to clone has a long stretch of A's in the middle of the coding sequence. Outcomes 1. The 5' part of the gene will be 5º missing. 2. The 3' part of the gene will be missing. An internal fragment of the gene will be missing. 4. The gene will be missing from the library.

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When making a cDNA our template is mRNA and the product is cDNA In A the templat... View the full answer