Question: RNA - seq libraries were constructed by NEBNext Poly ( A ) mRNA Magnetic Isolation Module ( NEB E 7 4 9 0 ) and
RNAseq libraries were constructed by NEBNext PolyA mRNA Magnetic Isolation Module
NEB E and NEBNext Ultra II NonDirectional RNA Second Strand Synthesis Module
NEB E mRNA was purified with polyT magnetic beads and first and second strand
cDNA was synthesized. The resulted cDNA was purified by AMPure XP beads : and eluted
in L nucleotidefree water. The subsequent procedures were the same as described in
ChIPseq library construction, except that the sequencing depth was million reads per
library. RNAseq libraries were sequenced by Illumina Novaseq platform with pairend reads
of bp
what feature of mRNA did the authors take advantage of to enrich for mRNA
Step by Step Solution
There are 3 Steps involved in it
1 Expert Approved Answer
Step: 1 Unlock
Question Has Been Solved by an Expert!
Get step-by-step solutions from verified subject matter experts
Step: 2 Unlock
Step: 3 Unlock
