Suppose a scientist identified two yeast mutants, called huh 1 and huh2. The mutants have constitutive expression
Question:
Suppose a scientist identified two yeast mutants, called huh 1 and huh2. The mutants have constitutive expression of the nop operon, which includes the genes nop 1 and nop 2. One of the mutants is a point mutation in a DNA- binding site, and the other mutant is a deletion of the gene which encodes the DNA-binding protein.
Which term describes the regulatory function of the DNA-binding protein?
Negative regulator
Not a regulator
Positive regulator
The scientist then performs an experiment mating the two haploid yeast together to determine which mutation is in the DNA-binding protein and which is in the DNA-binding site. The experimental setup is listed below, wherein a minus indicates a loss-of-function mutation, and a plus indicates the wild-type allele.
How will nop 1 and nop2 be expressed if huh 1 encodes the DNA-binding protein and huh2 is a mutation in the binding stie?
How will nop 1 and nop2 be expressed if huh 2 encodes the DNA-binding protein and huh 1 is a mutation in the binding site?
Expert Answer:
A negative regulator Since both the DNA binding site mutant and deletion of DNA binding protein lead... View the full answer
