Question: 1. (10 points) From a kinetics experiment, k, was determined to be 30sec!. For the kinetic assay, 0.15mL of a 0.05mg/mL solution of enzyme was

1. (10 points) From a kinetics experiment, k, was determined to be 30sec!. For the kinetic assay, 0.15mL of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 120,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (uM-min!) for the enzyme and catalytic efficiency ( in M-'sec'!) for the enzyme. The Km for the enzyme was determined to be 6.0 x 10 M

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