1. Draw a single Michaelis-Menten plot graph for the above data with an appropriate title (units)...
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1. Draw a single Michaelis-Menten plot graph for the above data with an appropriate title (units) and axis. 2. Draw a single Lineweaver-Burk plot graph. Please include additionally: a trend line for each set of data, values of the Y and X intercepts. 3. From question 2, Calculate the kinetic parameters with steps: V max, Km, Apparent V max and Km. 4. Calculate the relative constants with workings: a and a' 5. Therefore, identify the type of the inhibitor and explain. 6. Draw three Lineweaver-Burk plot: the uncompetitive, the mixed and the competitive inhibitor. Show one line for the enzyme without the inhibitor and one line for the enzyme with the inhibitor. Discuss the x-intercept and y-intercept values in each example and how they change/do not change with the addition of an inhibitor. What are the correlations of the intercepts with alpha and alpha' when an inhibitor is present? 7. Draw a Michaelis-Menten plot for allosteric enzyme with modulators. Add curves representing an allosteric activator and inhibitor. For each modulator, indicate if K0.5 value increase or decrease please. Thank you kinetic data [S] 0.00 0.25 0.50 1.00 2.00 4.00 7.00 12.00 20.00 Vo 0.00 0.59 1.11 2.00 3.33 5.00 6.36 7.50 8.33 Vo with I 0.00 0.23 0.42 0.71 1.11 1.54 1.84 2.07 2.22 1. Draw a single Michaelis-Menten plot graph for the above data with an appropriate title (units) and axis. 2. Draw a single Lineweaver-Burk plot graph. Please include additionally: a trend line for each set of data, values of the Y and X intercepts. 3. From question 2, Calculate the kinetic parameters with steps: V max, Km, Apparent V max and Km. 4. Calculate the relative constants with workings: a and a' 5. Therefore, identify the type of the inhibitor and explain. 6. Draw three Lineweaver-Burk plot: the uncompetitive, the mixed and the competitive inhibitor. Show one line for the enzyme without the inhibitor and one line for the enzyme with the inhibitor. Discuss the x-intercept and y-intercept values in each example and how they change/do not change with the addition of an inhibitor. What are the correlations of the intercepts with alpha and alpha' when an inhibitor is present? 7. Draw a Michaelis-Menten plot for allosteric enzyme with modulators. Add curves representing an allosteric activator and inhibitor. For each modulator, indicate if K0.5 value increase or decrease please. Thank you kinetic data [S] 0.00 0.25 0.50 1.00 2.00 4.00 7.00 12.00 20.00 Vo 0.00 0.59 1.11 2.00 3.33 5.00 6.36 7.50 8.33 Vo with I 0.00 0.23 0.42 0.71 1.11 1.54 1.84 2.07 2.22
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how to do it and what the results might indicate about the type of inhibitor present 1 MichaelisMenten Plot Title MichaelisMenten Plot of Kinetic Data Xaxis Substrate Concentration S in mM Yaxis Veloc... View the full answer
Related Book For
Managerial Economics
ISBN: 978-0133020267
7th edition
Authors: Paul Keat, Philip K Young, Steve Erfle
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