Question: (1) (ii) During the exponential phase a small but constant proportion of dead organisms was always observed. How can this observation be explained? (3 points)

 (1) (ii) During the exponential phase a small but constant proportion

(1) (ii) During the exponential phase a small but constant proportion of dead organisms was always observed. How can this observation be explained? (3 points) Use the data to obtain estimates of the maximum specific growth rate ilm, generation time, Og, net yield factor, Y and decay coefficient Kp. The organism dies exponentially. (13 points) (iii) If the saturation constant is 20 mg/l and the organism displays Monod kinetics, calculate the effluent biomass concentration from a chemostat culture of the organism in the same medium at dilution rates of 0.02 h, 0.6 h- and 1.1 h! Use the constants calculated in part (ii). Interpret your answers. (If you did not complete part (ii) carry out the calculation with what you consider to be reasonable estimates of the values of the constants). (10 points + 3 bonus points for perfect interpretation)

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