1. In the cloning procedure we used in the lab, it is possible, albeit unlikely, for...
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1. In the cloning procedure we used in the lab, it is possible, albeit unlikely, for multiple inserts to form a concatamer and be cloned into a single expression vector. If three egfp inserts were cloned into a single PET-41a expression vector, would EGFP be expressed (following IPTG induction), and if so, would these colonies have more fluorescence compared to clones that have one copy of the egfp gene (following IPTG induction)? 1. In the cloning procedure we used in the lab, it is possible, albeit unlikely, for multiple inserts to form a concatamer and be cloned into a single expression vector. If three egfp inserts were cloned into a single PET-41a expression vector, would EGFP be expressed (following IPTG induction), and if so, would these colonies have more fluorescence compared to clones that have one copy of the egfp gene (following IPTG induction)?
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PET41a expression vector is 5933 base pair in size So as lo... View the full answer
Related Book For
Income Tax Fundamentals 2013
ISBN: 9781285586618
31st Edition
Authors: Gerald E. Whittenburg, Martha Altus Buller, Steven L Gill
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