1 point MES (C6H13NO4S) is commonly used as a buffer in biology and biochemistry. It has a pKa of 6.15 at 20C. Calculate the concentration ratio of [HA][A] for an MES buffer with a pH of 7.44. Note: Although you will use different buffer systems and a different pH in lab, this is the same math that you will be using for lab. Report your answer to three places after the decimal. 1 point MES (C6H13NO4S) is commonly used as a buffer in biology and biochemistry. It has a SKa of 6.15 at 20C. Calculate the mass (in grams) of MES (HA in the Henderson-Hasselbalch equation) needed to obtain 100mL of a 0.100MMES buffer (total buffer concentration) with a pH of 9.97. Note: Although you will use different buffer systems and a different pH in lab, this is the same math that you will be using for lab. Report your answer to three places after the decimal