1. The quantity of organically bound nitrogen (org-N) released by acid digestion is referred to as Kjeldahl nitrogen. One method used to determine the
1. The quantity of organically bound nitrogen (org-N) released by acid digestion is referred to as Kjeldahl nitrogen. One method used to determine the Kjeldahl nitrogen content involves a back titration and is outlined below. a) A 10.00 mL sample is diluted to 100 mL with distilled water. b) A 25.00 mL aliquot of this diluted sample is pipetted into a digestion flask. Concentrated HSO4 and HO are added and the solution is heated for 45 mins (digestion). Under these conditions, the organic molecules are broken down and all the organic nitrogen is converted to NH4*. c) Concentrated NaOH is added to neutralize the excess HSO4 and to convert the NH4 to NH3, which is distilled into a flask containing 50.00 mL of 0.1011 N HSO4. d) The excess HSO4 was determined by titration with 5.12 mL of 0.1266 N NaOH. What is the mass of Kjeldahl nitrogen in the orignal sample in mg/L?
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