Question: 1. Total protein in an unknown sample was estimated using the Lowry and Bradford assays. Results were 33 +2 ug/mL from the Lowry assay and

1. Total protein in an unknown sample was estimated using the

1. Total protein in an unknown sample was estimated using the Lowry and Bradford assays. Results were 33 +2 ug/mL from the Lowry assay and 21 +1 ug/ mL from the Bradford assay, using stock solutions of bovine serum albumin (BSA) as standards in each assay. The unknown sample was then thoroughly oxygenated, and the assays were repeated. The Lowry method yielded 22+ 1 ug/mL, while the Bradford results did not change. Why did the results of the Lowry assay decrease by 33%

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