$1.$ Using the data $($DO versus time$)$ provided in Excel spreadsheet, calculate kla for oxygen at different agitation rates and at different gas flow rates. Make a plot of kLa against agitation rate and another plot showing kla against gas flow rate. If you use Rate method in addition to integral method, you will get extra point.

Kla Test, $2500$ L bloreactor, plitch impeller blades

Condition: $2$ LPM Flow, $300$ RPM

Kla Test, $2500$L bioreactor, pitch impeller blades Condition: $2$LPM Flow, $300$ RPM Time$($sec$)$ Time$($min$)$ DO$\%00\mathrm{.}051542\mathrm{.}6152995\mathrm{.}0254577\mathrm{.}63562810\mathrm{.}54582513\mathrm{.}855107317\mathrm{.}965138423\mathrm{.}175181330\mathrm{.}285250441\mathrm{.}795$ Condition: $2$LPM Flow, $375$ RPM Time$($sec$)$ Time$($min$)$ DO$\%00\mathrm{.}05951\mathrm{.}6151913\mathrm{.}2253085\mathrm{.}1354377\mathrm{.}3455889\mathrm{.}85579613\mathrm{.}365104317\mathrm{.}475133122\mathrm{.}285186031\mathrm{.}095$ Condition: $2$LPM$,450$ RPM Time$($sec$)$ Time$($min$)$ DO$\%00\mathrm{.}05751\mathrm{.}3151232\mathrm{.}1251762\mathrm{.}9352404\mathrm{.}0453175\mathrm{.}3554257\mathrm{.}1655949\mathrm{.}97593515\mathrm{.}685199133\mathrm{.}295$ Condition: $1\mathrm{.}5$ LPM$,375$ RPM Time$($sec$)$ Time$($min$)$ DO$\%00\mathrm{.}051362\mathrm{.}3152574\mathrm{.}3253936\mathrm{.}5355689\mathrm{.}54577312\mathrm{.}955105017\mathrm{.}565143123\mathrm{.}875207934\mathrm{.}785356859\mathrm{.}595$ Condition: $0\mathrm{.}7$ LPM$,375$ RPM Time$($sec$)$ Time$($min$)$ DO$\%00\mathrm{.}051592\mathrm{.}6153205\mathrm{.}3255288\mathrm{.}83577312\mathrm{.}945106617\mathrm{.}855146624\mathrm{.}465199133\mathrm{.}275260943\mathrm{.}585340556\mathrm{.}795$Time $($sec$)$ Time $($min$)$ DO$\%00\mathrm{.}051542\mathrm{.}6152995\mathrm{.}5254577\mathrm{.}63562810\mathrm{.}54582513\mathrm{.}855107317\mathrm{.}965138423\mathrm{.}175181330\mathrm{.}285250441\mathrm{.}795$

Condition:$2$LPM Flow, $375$ RPM

rime$($sec$)$ Time $($min$)$ DO$\%00\mathrm{.}05951\mathrm{.}6151913\mathrm{.}2253085\mathrm{.}1354377\mathrm{.}3455889\mathrm{.}85579613\mathrm{.}365104317\mathrm{.}475133122\mathrm{.}285186031\mathrm{.}095$

Condition: $2$LPM$,450$ RPM

IIme $($sec$)$ Time $($min$)$ DO$\%00\mathrm{.}05751\mathrm{.}3151232\mathrm{.}1251762\mathrm{.}9352404\mathrm{.}0453175\mathrm{.}3554257\mathrm{.}1655949\mathrm{.}97593515\mathrm{.}685199133\mathrm{.}295$

Condition:$1\mathrm{.}5$ LPM$,375$ RPM

rime $($sec$)$ Time $($min$)$ DO$\%00\mathrm{.}051362\mathrm{.}3152574\mathrm{.}3253936\mathrm{.}5355689\mathrm{.}54577312\mathrm{.}955105017\mathrm{.}565143123\mathrm{.}875207934\mathrm{.}785356859\mathrm{.}595$

Condition:$0\mathrm{.}7$ LPM$,375$ RPM

IIme $($sec$)$ Time $($min$)$ DO$\%00\mathrm{.}051592\mathrm{.}6153205\mathrm{.}3255288\mathrm{.}83577312\mathrm{.}945106617\mathrm{.}855146624\mathrm{.}465199133\mathrm{.}275260943\mathrm{.}585340556\mathrm{.}795$