1. You are given a 5.0 mg/mL protein standard solution, and asked to do a 5-step, 2-fold serial dilution to do a standard curve. What is the final concentration of protein standard in mg/mL? Record the correct amount, rounded to three decimal places.

2. You are given a 5.0 mg/mL protein standard solution, and asked to do a 5-step, 2-fold serial dilution to do a standard curve. After the third step of the dilution, what is the concentration of the protein standard, in mg/mL? Record the correct amount, rounded to two decimal places.

3. Your supervisor provides you with a 1.0 mg/mL solution of Bovine Serum Albumin (BSA), and asks you to make a 5-step 2-fold serial dilution. What would be the dilution ratio at step 3?