1. You are making 500 ml of Luria Bertani (LB) broth, and the ingredients are listed...

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1. You are making 500 ml of Luria Bertani (LB) broth, and the ingredients are listed as followed. Determine how many grams of each ingredient you will need. After you make the LB broth, you take 300 ml out of the 500 ml to make another special type of media. This special type of media is made by adding additional 1% Fetal Bovine Serum (FBS) and 3% histidine (His) to the original LB broth. % LB Broth (500 ml) Grams needed 0.5% yeast extract 0.75 % NaCl 1.0% tryptone LB (300 ml) + FBS + His 1% FBS 3% His Grams needed g g g g 1. You are making 500 ml of Luria Bertani (LB) broth, and the ingredients are listed as followed. Determine how many grams of each ingredient you will need. After you make the LB broth, you take 300 ml out of the 500 ml to make another special type of media. This special type of media is made by adding additional 1% Fetal Bovine Serum (FBS) and 3% histidine (His) to the original LB broth. % LB Broth (500 ml) Grams needed 0.5% yeast extract 0.75 % NaCl 1.0% tryptone LB (300 ml) + FBS + His 1% FBS 3% His Grams needed g g g g

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We must first ascertain the proportion of each ingredient in the finished product in order to calcul... View the full answer