1. You should understand the Michael's Menton equation: What does Km tell us about the enzyme What does Vmax tell us about the enzyme 2. Know the line-weaver Burke plot. Know what each part of the equation means. Know how to solve for Vmax or Km when the slope, x-intercept or y-intercept are given. 3a. Know how Vmax and Km are affected when each type of inhibitor is added to a non-allosteric enzymatic reaction: competitive inhibitor non-competitive inhibitor uncompetitive inhibitor mixed inhibitor 3b. How would the line-weaver burke plot for an enzymatic reaction change when each type of inhibitor is added to an enzymatic reaction. competitive inhibitor non-competitive inhibitor uncompetitive inhibitor mixed inhibitor 4. What is the Vmax if the x-intercept is 0.15L/mol and the slope is 0.75min/L ? 5. Using the graph below, calculate the Km for the enzymatic reaction