1.(16 points) Trosset chapter 12.6 problem set G.

Researchers compared antibody responses in normal and alloxan diabetic mice. Three groups of mice were studies: normal, alloxan diabetic, and alloxan diabetic treated with insulin. Several comparisons are of interest:

Does the antibody response of alloxan diabetic mice differ from the antibody response of normal mice?

Does treating alloxan diabetic mice with insulin affect their antibody response?

Does the antibody response of alloxan diabetic mice treated with insulin differ from the antibody response of normal mice?

Normal 156 282 197 297 116 127 119 29 253 122 349 110 143 64 26 86 122 455 655 14 Alloxan 391 46 469 86 174 133 13 499 168 62 127 276 176 146 108 276 50 73 Alloxan 82 100 98 150 243 68 228 131 73 18 + insulin 20 100 72 133 465 40 46 34 44 Table 12.7: Amounts of nitrogen-bound bovine serum albumin produced by normal and alloxan diabetic mice