2. (15 points) Consider the oligopeptide given below (written in the 3- letter code): Glu-His-Trp-Ser-Gly-Leu-Arg-Ala-Gly Answer...

Transcribed Image Text:

2. (15 points) Consider the oligopeptide given below (written in the 3- letter code): Glu-His-Trp-Ser-Gly-Leu-Arg-Ala-Gly Answer Parts A - D below: • Part A: Draw its full chemical structure as it would exist at pH = 8, then answer the questions below. You do not need to indicate stereochemistry, but make sure it is correct if you do. • Part B: Identify the net charge of the peptide at this pH. • Part C: Calculate the pl of this oligopeptide. Show your work. • Part D: Identify the results (you may express your answer(s) with either the 1-letter or 3-letter codes) of independently treating this oligopeptide with: o chymotrypsin o trypsin o endoproteinase Glu-C 2. (15 points) Consider the oligopeptide given below (written in the 3- letter code): Glu-His-Trp-Ser-Gly-Leu-Arg-Ala-Gly Answer Parts A - D below: • Part A: Draw its full chemical structure as it would exist at pH = 8, then answer the questions below. You do not need to indicate stereochemistry, but make sure it is correct if you do. • Part B: Identify the net charge of the peptide at this pH. • Part C: Calculate the pl of this oligopeptide. Show your work. • Part D: Identify the results (you may express your answer(s) with either the 1-letter or 3-letter codes) of independently treating this oligopeptide with: o chymotrypsin o trypsin o endoproteinase Glu-C