Question: 2. ( 3 pts.) An enzyme is known to require a 2mM concentration of Mg2+ for stability. If the Mg2+ is removed the protein is

2. ( 3 pts.) An enzyme is known to require a 2mM concentration of Mg2+ for stability. If the Mg2+ is removed the protein is irreversibly denatured. Suppose that you try to purify the enzyme with both ion-exchange and gel filtration (size exclusion) chromatography and in both cases the enzyme loses activity. Explain why this might happen and what modifications you could make to improve the situation

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