When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit...

 

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When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit at room temperature for 3 min, a yellow colour is seen to develop. Select the following option(s) that describe this situation. O A highly intense yellow colour indicates a high concentration of product in solution O A highly intense yellow colour indicates a high concentration of substate in solution. O Reaction catalysed by alkaline phosphatase is being monitored via disappearance of substrate. O Reaction catalysed by alkaline phosphatase is being monitored via appearanice of product. O Options (a) and (d) are both correct. When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit at room temperature for 3 min, a yellow colour is seen to develop. Select the following option(s) that describe this situation. O A highly intense yellow colour indicates a high concentration of product in solution O A highly intense yellow colour indicates a high concentration of substate in solution. O Reaction catalysed by alkaline phosphatase is being monitored via disappearance of substrate. O Reaction catalysed by alkaline phosphatase is being monitored via appearanice of product. O Options (a) and (d) are both correct.

Answer rating: 100% (QA)

In the reaction the ALP catalyzes the hydrolysis of the colorless organic phosphate ester substrate pNitrophenylphosphate to the yellow colored produc... View the full answer