Question: When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit at room temperature for 3 min, a yellow

When alkaline phosphatase is added to substrate in borate buffer (pH 9.5)

When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit at room temperature for 3 min, a yellow colour is seen to develop. Select the following option(s) that describe this situation. O A highly intense yellow colour indicates a high concentration of product in solution O A highly intense yellow colour indicates a high concentration of substate in solution. O Reaction catalysed by alkaline phosphatase is being monitored via disappearance of substrate. O Reaction catalysed by alkaline phosphatase is being monitored via appearanice of product. O Options (a) and (d) are both correct.

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In the reaction the ALP catalyzes the hydrolysis of the colorless organic phosphate ester substrate pNitrophenylphosphate to the yellow colored produc... View full answer

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