Question: 2. Now you need to prepare a different buffer: 2.000L of 2.00M phosphate buffer, pH 7.40. You have the following reagents available to you. (

2. Now you need to prepare a different buffer: 2.000L of

2. Now you need to prepare a different buffer: 2.000L of 2.00M phosphate buffer, pH 7.40. You have the following reagents available to you. ( pKa 's for phosphoric acid are: 2.14,7.20, and 12.40 ) a. From the table above, select the combination of reagents that you will use for your buffer. (Note: there are several possibilities - you just need to choose one.) (1) b. Calculate how much of each reagent (and water) you will use to make this buffer

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