$(2$ points$)$ List the $5$ necessary instrument components for HPLC analysis.

c$.$ Estimate the resolution of the DOM and FFA peaks. For this estimation,

use the width of the peak at the base $($a simplification for this case$).$

$(3$ points$)$ The quantification of singlet oxygen in water is achieved by following

the degradation of a chemical probe, in this case furfuryl alcohol. Below is a

chromatogram for the quantification of FFA in the presence of DOM. FFA elutes

at $3\mathrm{.}2$ minutes. Assume that the dead volume time is $1$min.

a$.$ What is the retention factor for the FFA peak? Compare this value to the

retention factor of the DOM peak observed at $1\mathrm{.}32$ minutes.

$k=\frac{t_R-t_m}{t_m}$

b$.$ What is the selectivity factor for the FFA peak compared to that of the

DOM peak?

$=\frac{t_{r,B}-t_m}{t_{r,A}-t_m}$