Question: 2. You continue studying the same Mg2+ protein from question 1. Its structure has been determined. The structure shows that it contains a Ser, Glu,
2. You continue studying the same Mg2+ protein from question 1. Its structure has been determined. The structure shows that it contains a Ser, Glu, and Thr residue in the first coordination sphere, and the second coordination sphere contains a Tyr, a Pro, and a His. You would like to create a variant that cannot bind metal using site-directed mutagenesis. a. Which residue(s) will you select to mutate and what will you mutate it(them) to? b. Explain your rationale (it may be useful to draw structures)
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