4. Choose the one answer that fits best. Which of the following statements regarding a hypothetical...
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4. Choose the one answer that fits best. Which of the following statements regarding a hypothetical bacterial transformation experiment using pGLO and E. coli is NOT correct? a. you would expect an LB/Amp plate containing E. coli (-)pGLO not to have any colonies you would expect an LB plate containing E. coli (-)pGLO to have a lawn of bacteria you would expect an LB/Amp plate containing E. coli (+)pGLO to fluorescence under UV light you would expect the LB/Amp/Ara plate containing E. coli (+)pGLO to have about the same amount of colonies as the LB/Amp plate containing E. coli (+)pGLO e. you would expect an LB plate containing E. coli (+)pGLO to have a lawn of bacteria 5. Choose the one answer that fits best. You performed a bacterial transformation on E. coli, using PGLO plasmid which contains a Green Fluorescent Protein gene under arabinose induced expression, and a gene coding for beta-lactamase. Your negative control has no colonies and your positive control has a lawn of bacteria. When you analyze your experimental plate under UV light, you see 62 whitish colonies but none fluoresce green. Which of the following procedural errors can explain this result? a. b. c. d. b. You plated E. coli (-)pGLO on this plate instead of E. coli (+)pGLO The plasmid was engineered wrong and contained no bla gene C. You used an LB/Amp plate for your experimental to plate E. coli (+)pGLO d. The arabinose was not added when the LB/Amp plate was poured e. Both c and d can explain this result Bacterial Transformation 145 4. Choose the one answer that fits best. Which of the following statements regarding a hypothetical bacterial transformation experiment using pGLO and E. coli is NOT correct? a. you would expect an LB/Amp plate containing E. coli (-)pGLO not to have any colonies you would expect an LB plate containing E. coli (-)pGLO to have a lawn of bacteria you would expect an LB/Amp plate containing E. coli (+)pGLO to fluorescence under UV light you would expect the LB/Amp/Ara plate containing E. coli (+)pGLO to have about the same amount of colonies as the LB/Amp plate containing E. coli (+)pGLO e. you would expect an LB plate containing E. coli (+)pGLO to have a lawn of bacteria 5. Choose the one answer that fits best. You performed a bacterial transformation on E. coli, using PGLO plasmid which contains a Green Fluorescent Protein gene under arabinose induced expression, and a gene coding for beta-lactamase. Your negative control has no colonies and your positive control has a lawn of bacteria. When you analyze your experimental plate under UV light, you see 62 whitish colonies but none fluoresce green. Which of the following procedural errors can explain this result? a. b. c. d. b. You plated E. coli (-)pGLO on this plate instead of E. coli (+)pGLO The plasmid was engineered wrong and contained no bla gene C. You used an LB/Amp plate for your experimental to plate E. coli (+)pGLO d. The arabinose was not added when the LB/Amp plate was poured e. Both c and d can explain this result Bacterial Transformation 145
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b you would expect an LB plate containing E coli pGLO to have a lawn of bacteria The other statement... View the full answer
Related Book For
Applied Regression Analysis and Other Multivariable Methods
ISBN: 978-1285051086
5th edition
Authors: David G. Kleinbaum, Lawrence L. Kupper, Azhar Nizam, Eli S. Rosenberg
Posted Date:
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