5.. Ionization, pka and pl. Given a polypeptide with N side chain groups that can be protonated and deprotonoated with known pka's (pKa1, pka2, ... pkan), one can estimate or calculate its pl (isoelectric point), which is the pH at which the net charge of the molecule is zero. For a molecule with 2 ionizable groups (pKai and pka2), the pl is given by pK, +p2 pl = 2 (a) At what pH the net charge on the free amino acid lysine is zero (i.e. it is neutral)? (b) What is the estimated net charge on the short peptide: MEGKRLVCH at pH 7.0? Explain your answers