Question: 6. (1 point) Your colleague isolates a plasmid DNA sample from bacteria. You run gel electrophoresis to analyze the plasmid. Your gel appears as follows:
6. (1 point) Your colleague isolates a plasmid DNA sample from bacteria. You run gel electrophoresis to analyze the plasmid. Your gel appears as follows: Your colleague tells you that there was enly one plasmid species in the sample. Assuming they are correct, explain why there are multiple bands. 7. (2 points) Using molecular cloning techniques, you inserted a fragment of DNA into a plasmid. To confirm that you obtained the right product, you decide to sequence the DNA. You design an appropriate sequencing primer (Primer. 5'-CTGTGACG-3'). You conduct Sanger sequencing reactions and obtain the following gel. What is the sequence of the unknown DNA fragment? (Answer format: 5'-NNNNNN...NNNNNN-3')
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