8. A membrane-bound compartment contains a macromolecule X with a net charge of -3 . The membrane is not permeable to X, but is permeable to Na+,K+and Cl. The known concentrations of ionic species are: [X3]in=50mM,[Na+]in=20mM,[Na+]out=80mM, and [Cl]out=100mM. Calculate [K+]in,[K+]out, and [Cl]in and determine the potential across the membrane. Assume that Na+,K+,Cland X3 are the only charged species present. (15pt) 3 TABLE 2.3.2 Ionic Properties at Infinite TABLE 2.3.1 Cation Transference Numbers for Aqueous Solutions at 25Ca a From D. A. MacInnes, "The Principles of Electrochemistry," Dover, New York, 1961, p. 85 and references cited therein. Dover, New York, 1961, p. 342 b Moles of positive (or negative) charge per liter. b Calculated from 0