Question: 96 mL and 100 mL are both incorrect Calculate the volume of each stock solution needed to prepare 1.0L of the 0.10 M HEPES buffer

96 mL and 100 mL are both incorrect

Calculate the volume of each stock solution needed to prepare 1.0L of the 0.10 M HEPES buffer at a pH = 8.0. volume HA (ml) - 1 100 volume A (ml) = 100 (Tolerance is +/-2%) One way to prepare the HEPES buffer involves preparing solutions of the sodium salt form of HEPES (A) and the weak acid form of HEPES (HA) and then combining the solutions to obtain a buffer with the desired pH and concentration. Calculate the volume of each stock solution needed to prepare 1.0L of the 0.10 M HEPES buffer at a pH = 8.0. volume HA (ml) - 1 100 volume A (ml) = 100 (Tolerance is +/-2%) One way to prepare the HEPES buffer involves preparing solutions of the sodium salt form of HEPES (A) and the weak acid form of HEPES (HA) and then combining the solutions to obtain a buffer with the desired pH and concentration

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

The cable shown is subjected to the uniform load w 0 . Determine the ratio between the rise h and the span L that will result in using the minimum amount of material for the cable. Wo

Please help. I uploaded the picture of what the exercise is asking as well as the data we obtained after doing the experiment. This is all the information I have. Thank you! Time (sec) Absorbance...

I need help with this I would appreciate the help Use the concentration in the cuvette (assay concentration) that you obtained in Question 7 to calculate the concentration of the unknown sample that...

ASSIGNMENT 1 - Lab Calculations - Duo Tuesday, January 26, 2022 @ 11:59 PM DNA Gel Electrophoresis Butler Preparation About Buffers Solid reagents are typically dissolved in a volume of delonized or...

K=1.38100.10 Mydlo 0 :0 00 IT EN-C-H 11 HH pk, carboxylic acid = 2.34 pk, protonated amine 9.6 15. One form of the amino acid glycine is shown above. At which would this be the predominant form of...

Post-Lab questions and calculations to submit next week: 1. In the boxes below, write how many uls the following digital readout correspond to in each of the pipetters? Digital Readout 0 7 0 P20 P200...

Solution A Solution B Kinetic Trial Deionized Water Buffer Starch 0.1 MH:0,*** 1 2 40 ml 3.0 ml 2.0 ml. 10 ml. 20 ml. 0.0 ml 5.0 ml 100 ml 100 ml 1.00 ml 100 ml 1.00 ml 1.00 ml. 100 ml 1.00 ml 0.3....

Not sure why I got these incorrect A 1.38 L buffer solution consists of 0.285 M propanoic acid and 0.106 M sodium propanoate. Calculate the pH of the solution following the addition of 0.073 mol HCl....

Can you please help me with the steps to answers the questions bellow thank you so much DETERMINATION OF THE DISSOCIATION CONSTANT OF WEAK ACIDS The acids used in parts (a) and (b) are not...

please answer all questions. Procedure is Experiment: Chymotrypsin Catalysis Prelab Assignment 1. 12 points) The table provided in Step 1 of Day 1's procedure outlines four different experimental...

Buffer Solution 1. 67.5 g of ammonium chloride was dissolved in 509 mL of 28% ammonium solution. Once the ammonium chloride was completely dissolved, 5.0 g EDTA-Na salt was added. The resulting...

Instructions: Determine the free cash flow for 2020 Presented below is a condensed version of the comparative statements of financial position for BMW Company. for the last 2 years at December 31...

Give the names of the following ions. a. Mn2+ b. Ni2+ c. Co2+ d. Co3+

uestion 1 ( 1 point ) Saved A tax return preparer may disclose information obtained from the taxpayer without being subject to civil and criminal penalty if such disclosure is: a ) for other than the...

using Java, please answer as quickly ! Consider the following recursive definition for the Ackermann function: 20 { n+1 if m=0 A(m, n) Am - 1,1) ifm > O and no Afm - 1, Am, n-1)) ifm > 0 and n > 0....

Ultimately, the market unravels because buyers are risk-averse. If buyers were riskneutral, there would always be prices at which cars would sell.

The Akerlof model indicates that government intervention is the only way to solve the adverse selection problem.

and 100. Suppose further that sellers utility is given by US =M+a n i Xi where M is the number of units of video games, which sell at $1 per game, and a is a utility function parameter that is...