Question: A 1 5 - m L sample is pipetted directly into a 3 0 0 - m L incubation bottle. The initial D O of

A 15-mL sample is pipetted directly into a 300-mL incubation bottle. The initial DO of the diluted
sample is 8.4mgL, and its final DO after the 5-day test is 1.8mgL. The seeded dilution water is
separately incubated in a 300-mL bottle for 5 days, and the initial and final DO concentrations are,
respectively, 8.8mgL and 7.5mgL. After a long period of time, the sample DO is 0.5mgL, and it
no longer seems to be dropping. The dilution water DO is reduced to 7.0mgL. Assume nitrification
has been inhibited so the only BOD being measured is carbonaceous.
a. If the sample and the seeded dilution water are both incubated at 20C for 5 days, what is the
BOD5 of the sample?
b. Assuming no nitrification effects, estimate the ultimate carbonaceous BOD (L0).
c. What would be the remaining BOD after five days have elapsed (L5)?
d. Estimate the deaeration reaction rate constant day {:?-1).
A 1 5 - m L sample is pipetted directly into a 3

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