Question: A 5 - day BOD test is performed on a sample of chlorinated wastewater effluent. Sixty milliliters of wastewater sample and 2 ml of seed

A 5-day BOD test is performed on a sample of chlorinated wastewater effluent. Sixty milliliters of
wastewater sample and 2 ml of seed are added to 300-ml BOD bottles and mixed with enough dilution
water to fill the bottles. Seed must be added, since wastewater effluent is chlorinated, which kills
pathogens and bacteria. For the blanks or controls, 2 ml of seed are added to 300-ml BOD bottles and
mixed with enough dilution water to fill the bottles. The average dissolved-oxygen concentrations of the
diluted wastewater samples and blanks on day 1 of the test were 6.8 and 7.4 mg/L, respectively. After
incubation at 20C for 5 days, the average DO concentrations of the diluted wastewater samples and
blanks were 2.5 and 7.1 mg/L, respectively. Calculate the 5-day BOD of the chlorinated effluent.

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