a. A 250-mL stock HCl solution is placed in a volumetric flask and diluted to a total volume of 1.00 L. The concentration of the diluted specimen is 2.00 M. What is the concentration of the stock HCl?

b. A 250-mL 45.0% ethanol solution is needed. In the stock room is a bottle of 75.0% ethanol. How much of the 75.0% ethanol should you use to prepare the 250-mL solution?

c. A 125-mL, 3.50M solution is diluted to 500 mL. What is the concentration of the new solution? 6.

Spectrophotometry

a. Given the absorbance of a 150 mg/dl standard is 0.425, and the absorbance of QC 1 is 0.205, calculate the concentration of QC 1.

b. Given the absorbance of a 250 mg/dl standard is 0.750, and the absorbance of QC 2 is 0.680, calculate the concentration of QC 2. 7. Miscellaneous a. A 43-yr-old man with a family history of CAD has the following lipid profile. Calculate the LDL cholesterol. All results in mg/dl: Total Cholesterol= 280 Triglycerides= 150 HDL= 50 b. Given the following results in mg/dl, calculate the LDL. Total Cholesterol = 250, Triglycerides = 475, HDL = 40.

c. A 24-hr urine was collected for a creatinine clearance test. TV 1800 ml, plasma creatinine 2.4 mg/dl, urine creatinine 167 mg/dl. The patient is 6 feet 4 inches and 380 pounds (2.56 meters squared). Calculate the creatinine clearance.

d. Calculate the creatinine clearance of a 24-hr urine with a total volume of 2.1 L. The plasma creatinine is 1.8 mg/dl and the urine creatinine is 155 mg/dl.

e. A 12-hr urine collection for creatinine clearance has a total volume of 0.850 L, plasma creatinine of 1.1 mg/dl, and urine creatinine of 117 mg/dl. What is the CCl?

f. Calculate the MCH given a hemoglobin of 14.0 g/dl and a RBC count of 5.1 x106 uL.

g. A CBC with manual differential was ordered for a premature newborn. 26 NRBC were counted in the differential. The babys WBC count is 27.5 x 103 uL. What is the corrected WBC count?

h. A WBC count was performed using a hemacytometer in a 1:20 diluted specimen. There are 140 WBCs counted in the 4 large Squares. What is the WBC count?

i. A sperm count was performed using a 1:20 dilution. An average of 72 sperm cells was counted in five small squares within the center square. What is sperm count per milliliters?

j. A reticulocyte count was performed and 45 reticulocytes were counted per 1000 RBC. What is the retic count?

k. Calculate the expected hemoglobin concentration if a patient had a Hct = 15% and pre-transfusion Hgb = 5 g/dl, and was given 3 units of packed RBC.

l. Calculate the expected hematocrit in a patient with a pretansfusion hematocrit =16% and hemoglobin of 6 g/dl if given 3 units of packed RBC.

m. A woman was rushed to the ER hemorrhaging as a result of a miscarriage. Her blood type is B Neg and the father is A Pos. A Kleihauer-Betke acid elution test determined the percentage of fetal cells in the mothers circulation to be 3.2%. How many units of RhIG should the woman be given to prevent the formation of D antibodies?

n. Calculate the SD with the following information:

Mean = 144 Sum of the squared differences = 56 n-1 = 9

o. For a hematocrit method, given a SD = 2.0 and a mean of 45. What is the Coefficient of Variation?

p. Calculate the serum osmolality with the following values: Na= 135 K= 3.5 Glucose= 205 BUN= 12