(a) An enzyme catalyzed reaction that involves two substrates gave the kinetic data in the table...

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(a) An enzyme catalyzed reaction that involves two substrates gave the kinetic data in the table below at a fixed enzyme concentration of 100 nM. Use the data to determine if the enzyme follows a ping-pong or sequential mechanism, and determine all associated KM and Vmax values. [25] 40 mM 200 mM [S]o v ([S2]o = 50 µM) (mol s1) v ([S2]o = 100 uM) v ([S2]o = 250 µM) v ([S2]o = 1 mM) 10 mM 20 mM 0.760 1.025 1.301 0.300 0.440 0.570 0.714 0.335 0.360 0.374 %3D 0.518 0.839 0.919 0.581 %3D 0.618 1.504 %3D (b) Determine the enzyme's catalytic efficiency. [3] (c) If you were going to develop an inhibitor for this reaction, which step would you target, based on its kinetics? [3] (a) An enzyme catalyzed reaction that involves two substrates gave the kinetic data in the table below at a fixed enzyme concentration of 100 nM. Use the data to determine if the enzyme follows a ping-pong or sequential mechanism, and determine all associated KM and Vmax values. [25] 40 mM 200 mM [S]o v ([S2]o = 50 µM) (mol s1) v ([S2]o = 100 uM) v ([S2]o = 250 µM) v ([S2]o = 1 mM) 10 mM 20 mM 0.760 1.025 1.301 0.300 0.440 0.570 0.714 0.335 0.360 0.374 %3D 0.518 0.839 0.919 0.581 %3D 0.618 1.504 %3D (b) Determine the enzyme's catalytic efficiency. [3] (c) If you were going to develop an inhibitor for this reaction, which step would you target, based on its kinetics? [3]