The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase:
CO2 + H2O → HCO-3 + H+
[H. De Voe and G. B. Kistiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, determine KM and Vmax for the reaction.
Carbon Dioxide Concentration 1/Velocity
(mmol L -1 (M-1 sec)
1.25.............. 36 × 103
2.5............... 20 × 103
5.0............... 12 × 103
20.0.............. 6 × 103