A biology 207 student sub cloned the promoterless, GFP gene from pCam-GFP into pUC18-207 using a...

   

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A biology 207 student sub cloned the promoterless, GFP gene from pCam"-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5AE. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem. Pfl108 I 1715 Bsa l 1766 Sal 12177 AmpR Xmn I 2294 AlwN I 1217 ORI PUC18-207 -2700 bp Ssp1 2501 Afl III 806 lacZ' promoter Sap I 683 Nde I 183 MCS 454 396 EcoR I Kpn I Bam HI Xbal Sal l Pst I Hind III Sac I Not! Which two restriction enzymes did the student use? Select the correct two answers. (2 marks, all or none) Select all that apply: A. Bam HI cross out B. Hind III cross out cross out OC. Sac I D. Sal I cross out E. Pst I cross out F. Not I cross out cross out O G. Xho I H. EcoR I cross out cross out O I. Xba I A biology 207 student sub cloned the promoterless, GFP gene from pCam"-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5AE. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem. Pfl108 I 1715 Bsa l 1766 Sal 12177 AmpR Xmn I 2294 AlwN I 1217 ORI PUC18-207 -2700 bp Ssp1 2501 Afl III 806 lacZ' promoter Sap I 683 Nde I 183 MCS 454 396 EcoR I Kpn I Bam HI Xbal Sal l Pst I Hind III Sac I Not! Which two restriction enzymes did the student use? Select the correct two answers. (2 marks, all or none) Select all that apply: A. Bam HI cross out B. Hind III cross out cross out OC. Sac I D. Sal I cross out E. Pst I cross out F. Not I cross out cross out O G. Xho I H. EcoR I cross out cross out O I. Xba I

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pUC18207 upon digestion with SalI gives two fragments One o... View the full answer