12. A drug company has developed two compounds that are inhibitors to a particular enzyme (we?ll call it ?taterase?). ?Inhibition of Taterase is known to reduce feelings of tension and stress in individuals with type A personality.?

Testing of the two drugs (inhibitors A and B) produced the kinetic data below. The concentrations of the inhibitors in the assays were 5.0 mM for inhibitor A and 0.1 mM for inhibitor B. ?The assays were done using with an enzyme present in solution at 0.1 ?M.

Make Lineweaver Burke plots of the data and determine they type of inhibition displayed with each inhibitor and Vmax, Km, kcat, and KI?values.

Based on your results, explain which drug you think would be most effective.

?Use Graph on Next Page

	Vo?(mol/min)
[substrate] mM	w/o ?I	w/ IA	w/ IB
0.5000	19.58	15.29	9.30
0.2500	12.32	9.50	6.30
0.1250	8.04	5.16	3.20
0.0625	4.41	2.91	1.70
0.0313	2.43	1.50	0.98

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